Fluorescence based real time monitoring of fouling in process chromatography
نویسندگان
چکیده
A real time monitoring of fouling in liquid chromatography has been presented. The versatility of the approach has been proven by successful implementation in three case studies with an error <1%. The first application demonstrates the monitoring of protein A ligand density and foulant concentration for assessing performance of protein A chromatography resin during purification of monoclonal antibodies. The observations have been supported from LC-MS/MS studies that were independently performed. The second application involves monitoring of foulant deposition during multimode cation exchange chromatography based purification of human serum albumin. Finally, in the third application, monitoring of foulants during multimodal hydrophobic interaction chromatography of recombinant human granulocyte colony stimulating factor is demonstrated. In all three cases, it is observed that the fluorescence intensity consistently increases with resin reuse as more foulants are deposited over time. The proposed approach can be readily used for real time monitoring of fouling and process control.
منابع مشابه
Online Fault Detection and Isolation Method Based on Belief Rule Base for Industrial Gas Turbines
Real time and accurate fault detection has attracted an increasing attention with a growing demand for higher operational efficiency and safety of industrial gas turbines as complex engineering systems. Current methods based on condition monitoring data have drawbacks in using both expert knowledge and quantitative information for detecting faults. On account of this reason, this paper proposes...
متن کاملA Statistical Method for Sequential Images – Based Process Monitoring
Today, with the growth of technology, monitoring processes by the use of video and satellite sensors have been more expanded, due to their rich and valuable information. Recently, some researchers have used sequential images for image defect detection because a single image is not sufficient for process monitoring. In this paper, by adding the time dimension to the image-based process monitorin...
متن کاملFrequency of Human Herpesvirus 6 (HHV-6) in Pterygium Using Real-Time PCR Based on SYBR-Green I Fluorescence
ABSTRACT Background and objectives: Pterygium is a non-cancerous growth of conjunctival tissue that can extend onto the corneal surface. The presence of some oncogenic viruses in pterygium and the neoplastic nature of these lesions led us to the postulated involvement of the viruses in the etiology of pterygium. Given the association of human herpesvirus 6 (HHV-6) with ocular disea...
متن کاملAssessment of BAM with ANP Approach; Case Study: Bank Sepah
In today's business environment in which coordination and adaptation with constant changes are the only ways of survival, real-time monitoring of activities and making the decisions accordingly are necessary. Since performance measurement cannot be managed independent of business processes, Business Activity Monitoring (BAM) systems should monitor performance metrics based on business processes...
متن کاملOptimization of a Membrane Filtration Process for Drinking Water Treatment Using Fluorescence-Based Measurements
Membrane fouling control is of paramount importance for sustainable operation of membranebased drinking water treatment processes. Natural organic matter (NOM) is considered as the major membrane foulant and therefore its characterization is important for implementing fouling control strategies. This study proposes a fluorescence-based modeling approach for estimating and predicting the fouling...
متن کامل